pBlueBac4.5/V5-His/CAT Sequence Range: 1 to 5703 Enzyme #Cuts Positions AatI 1 868 AatII 4 946 1555 2361 4782 AccI 1 960 AccIII 2 98 400 Acc16I 2 3057 5259 Acc65I 1 163 Acc113I 2 821 2799 AccB1I 6 163 2077 3327 4601 5205 5217 AccB2I 5 1234 3928 4509 4946 5240 AccB7I 1 636 AccBSI 3 2438 4239 4796 AcsI 7 404 1015 1115 1293 1359 1789 4901 AcyI 6 943 1552 1752 2358 2740 4779 AfeI 1 1232 AflIII 5 911 1625 1775 1880 4168 AgeI 1 917 AhdI 1 3280 Alw21I 5 148 2115 2612 2697 3858 Alw26I 9 628 905 950 2061 2243 2285 2438 3214 4770 Alw44I 3 2111 2608 3854 AlwNI 1 3759 Ama87I 2 147 167 AocI 1 5174 Aor51HI 1 1232 ApaLI 3 2111 2608 3854 ApoI 7 404 1015 1115 1293 1359 1789 4901 AseI 2 1759 3105 AsnI 2 1759 3105 Asp700I 2 1815 2680 Asp718I 1 163 AspEI 1 3280 AspHI 5 148 2115 2612 2697 3858 AspS9I 8 1183 2300 2916 3138 3155 3234 5287 5589 AvaI 2 147 167 AvaII 4 1183 2916 3138 5589 AviII 2 3057 5259 AxyI 1 5174 BalI 1 671 BamHI 1 138 BanI 6 163 2077 3327 4601 5205 5217 BanII 1 148 BanIII 1 4575 BbiII 6 943 1552 1752 2358 2740 4779 BbsI 1 1733 Bbv12I 5 148 2115 2612 2697 3858 Bbv16II 1 1733 BcnI 7 168 169 2208 2243 2744 3095 3791 BcoI 2 147 167 BfaI 9 133 174 949 1050 1234 1504 3087 3422 3675 BfmI 5 155 3034 3712 3903 5009 BglI 2 3162 5252 BglII 1 151 Bme18I 4 1183 2916 3138 5589 BmyI 6 148 1862 2115 2612 2697 3858 BpiI 1 1733 BpmI 5 526 3211 4676 4824 5174 Bpu14I 1 857 BpuAI 1 1733 BsaI 2 905 3214 Bsa29I 1 4575 BsaAI 1 1274 BsaBI 1 28 BsaHI 6 943 1552 1752 2358 2740 4779 BsaJI 10 167 189 635 636 705 895 1133 4008 5065 5355 BsaMI 3 397 804 1045 BsaOI 6 2762 2911 3834 4258 4554 5280 BsaWI 6 98 400 917 2984 3815 3962 BscI 1 4575 Bse8I 1 28 Bse21I 1 5174 Bse118I 2 917 3195 BseAI 2 98 400 BseCI 1 4575 BseDI 10 167 189 635 636 705 895 1133 4008 5065 5355 BseRI 2 884 1123 BsgI 3 1919 4277 4487 Bsh1285I 6 2762 2911 3834 4258 4554 5280 Bsh1365I 1 28 BshNI 6 163 2077 3327 4601 5205 5217 BsiEI 6 2762 2911 3834 4258 4554 5280 BsiHKAI 5 148 2115 2612 2697 3858 BsiMI 2 98 400 BsmI 3 397 804 1045 BsmBI 5 628 950 2243 2285 4770 BsmFI 3 100 1680 5168 BsoBI 2 147 167 Bsp13I 2 98 400 Bsp19I 2 189 705 Bsp106I 1 4575 Bsp119I 1 857 Bsp143II 5 1234 3928 4509 4946 5240 Bsp1286I 6 148 1862 2115 2612 2697 3858 Bsp1407I 1 1604 BspCI 3 2911 4554 5280 BspDI 1 4575 BspEI 2 98 400 BspHI 3 2335 2440 3448 BspLU11I 1 4168 BspMI 1 4623 BspXI 1 4575 BsrBI 3 2438 4239 4796 BsrBRI 1 28 BsrDI 3 423 3046 3220 BsrFI 2 917 3195 BsrGI 1 1604 BssAI 2 917 3195 BssSI 4 2304 2611 3995 4340 BssT1I 2 189 705 BstBI 1 857 Bst2BI 4 2304 2611 3995 4340 BstDEI 10 625 863 2118 2353 2779 3319 3485 3894 4893 5174 BstDSI 3 189 705 5065 BstH2I 5 1234 3928 4509 4946 5240 BstMCI 6 2762 2911 3834 4258 4554 5280 BstSFI 5 155 3034 3712 3903 5009 BstSNI 1 1274 BstX2I 9 138 151 2633 2650 3418 3430 3516 3527 5391 BstYI 9 138 151 2633 2650 3418 3430 3516 3527 5391 Bsu15I 1 4575 Bsu36I 1 5174 CbiI 1 857 CfrI 3 669 2887 4926 Cfr9I 1 167 Cfr10I 2 917 3195 Cfr13I 8 1183 2300 2916 3138 3155 3234 5287 5589 ClaI 1 4575 Csp45I 1 857 CvnI 1 5174 DdeI 10 625 863 2118 2353 2779 3319 3485 3894 4893 5174 DraI 6 324 663 1364 2702 3394 3413 DraII 1 2300 DrdI 2 2197 4066 DsaI 3 189 705 5065 EaeI 3 669 2887 4926 Eam1104I 3 1100 2481 5297 Eam1105I 1 3280 EarI 3 1100 2481 5297 Ecl136II 1 146 EclHKI 1 3280 Eco24I 1 148 Eco31I 2 905 3214 Eco32I 1 3 Eco47I 4 1183 2916 3138 5589 Eco47III 1 1232 Eco57I 4 1124 2614 3626 4669 Eco64I 6 163 2077 3327 4601 5205 5217 Eco81I 1 5174 Eco88I 2 147 167 Eco105I 1 1274 Eco130I 2 189 705 Eco147I 1 868 Eco255I 2 821 2799 EcoICRI 1 146 EcoO109I 1 2300 EcoRI 1 404 EcoRV 1 3 EcoT14I 2 189 705 EcoT38I 1 148 ErhI 2 189 705 Esp3I 5 628 950 2243 2285 4770 Esp1396I 1 636 FauI 9 389 833 1819 1833 1981 2173 2183 5278 5600 FriOI 1 148 FspI 2 3057 5259 GsuI 5 526 3211 4676 4824 5174 HaeII 5 1234 3928 4509 4946 5240 HgaI 9 1174 1334 1760 2190 2748 3478 4056 4556 4985 HgiEI 4 1183 2916 3138 5589 Hin1I 6 943 1552 1752 2358 2740 4779 HincII 3 961 4351 4975 HindII 3 961 4351 4975 HindIII 1 860 HpaI 2 4351 4975 Hsp92I 6 943 1552 1752 2358 2740 4779 KpnI 1 167 Kpn2I 2 98 400 Ksp632I 3 1100 2481 5297 LspI 1 857 MaeI 9 133 174 949 1050 1234 1504 3087 3422 3675 MamI 1 28 MfeI 2 1263 1608 MflI 9 138 151 2633 2650 3418 3430 3516 3527 5391 MluI 1 911 MluNI 1 671 MroI 2 98 400 MscI 1 671 MslI 4 2509 2868 3027 5109 Msp17I 6 943 1552 1752 2358 2740 4779 MspA1I 10 160 304 748 2178 2644 3585 3830 4607 5309 5425 MunI 2 1263 1608 Mva1269I 3 397 804 1045 NciI 7 168 169 2208 2243 2744 3095 3791 NcoI 2 189 705 NheI 1 132 NspI 3 2255 4172 5489 NspV 1 857 NspBII 10 160 304 748 2178 2644 3585 3830 4607 5309 5425 PaeR7I 1 147 PflMI 1 636 PinAI 1 917 PleI 6 965 1090 1607 3289 3792 4277 Ple19I 3 2911 4554 5280 Pme55I 1 868 PshBI 2 1759 3105 Psp1406I 3 491 2678 3051 PspAI 1 167 PspALI 1 169 Psp124BI 1 148 PstI 1 159 PstNHI 1 132 PvuI 3 2911 4554 5280 PvuII 4 160 304 5309 5425 RcaI 3 2335 2440 3448 SacI 1 148 SalI 1 959 Sau96I 8 1183 2300 2916 3138 3155 3234 5287 5589 ScaI 2 821 2799 SduI 6 148 1862 2115 2612 2697 3858 SfcI 5 155 3034 3712 3903 5009 Sfr274I 1 147 SfuI 1 857 SinI 4 1183 2916 3138 5589 SmaI 1 169 SmiI 1 1364 SnaBI 1 1274 SseBI 1 868 SspI 5 95 716 1368 1565 2475 SspBI 1 1604 SstI 1 148 StuI 1 868 StyI 2 189 705 SwaI 1 1364 TfiI 9 754 905 1237 4194 4360 4492 5059 5412 5645 Tsp45I 10 2231 2807 3018 4342 4544 4657 4780 4834 5102 5368 TspRI 6 2861 3760 3773 4279 5414 5699 Van91I 1 636 VneI 3 2111 2608 3854 VspI 2 1759 3105 XbaI 1 173 XhoI 1 147 XhoII 9 138 151 2633 2650 3418 3430 3516 3527 5391 XmaI 1 167 XmaCI 1 167 XmnI 2 1815 2680 The enzymes below do not restrict this vector. AclNI AflII ApaI AscI AspI AtsI AvrII BbeI BbrPI BbuI BclI BfrI BlnI BlpI Bpu1102I BsePI BsiWI Bsp68I Bsp120I Bsp1720I BspTI BssHII Bst98I Bst1107I BstEII BstPI BstXI BstZI CciNI CelII Cfr42I CpoI CspI DraIII EagI EclXI Eco52I Eco72I Eco91I EcoNI EcoO65I EcoT22I EheI FauNDI FbaI FseI KasI KspI Ksp22I Mph1103I MroNI MspCI NaeI NarI NdeI NgoAIV NgoMI NotI NruI NsiI PacI PaeI PauI Pfl23II PmaCI PmeI PmlI Ppu10I PpuMI PshAI Psp5II PspEI PspLI PspOMI RsrII SacII SapI SbfI SexAI SfiI Sfr303I SgfI SgrAI SpeI SphI SplI SrfI Sse8387I SstII SunI Tth111I Vha464I XcmI XmaIII Zsp2I