(Linear) (Circular) MAPSORT of: pCDM8.seq check: 9832 from: 1 to: 4356 With 203 enzymes: * February 23, 1996 11:38 .. AatII G_ACGT'C Cuts at: 1682 1735 1818 2004 1682 Size: 53 83 186 4034 Fragments arranged by size: 4034 186 83 53 AccI GT'mk_AC Cuts at: 2367 3663 3692 2367 Size: 1296 29 3031 Fragments arranged by size: 3031 1296 29 AceIII CAGCTCnnnnnnn'nnnn_ Cuts at: 667 3576 667 Size: 2909 1447 AciI C'CG_C Cuts at: 34 43 178 288 409 428 555 820 974 Size: 9 135 110 121 19 127 265 154 Cuts at: 974 1001 1029 1032 1046 1115 1179 1181 1209 Size: 27 28 3 14 69 64 2 28 Cuts at: 1209 1236 1293 1615 1643 1655 1669 1836 1927 Size: 27 57 322 28 12 14 167 91 Cuts at: 1927 1960 2064 2085 2239 2393 2446 2483 2549 Size: 33 104 21 154 154 53 37 66 Cuts at: 2549 2579 2639 2643 3355 3415 3477 3710 4025 Size: 30 60 4 712 60 62 233 315 Cuts at: 4025 4179 4191 4200 4212 4222 4233 4279 34 Size: 154 12 9 12 10 11 46 111 Fragments arranged by size: 712 322 315 265 233 167 154 154 154 154 135 127 121 111 110 104 91 69 66 64 62 60 60 57 53 46 37 33 30 28 28 28 27 27 21 19 14 14 12 12 12 11 10 9 9 4 3 2 AflIII A'CryG_T Cuts at: 1535 1535 Size: 4356 AluI AG'CT Cuts at: 69 326 462 587 591 598 657 917 1121 Size: 257 136 125 4 7 59 260 204 Cuts at: 1121 1170 1442 2123 2198 2554 2725 3054 3235 Size: 49 272 681 75 356 171 329 181 Cuts at: 3235 3590 3638 3674 3745 3848 3982 4295 4349 Size: 355 48 36 71 103 134 313 54 Cuts at: 4349 69 Size: 76 Fragments arranged by size: 681 356 355 329 313 272 260 257 204 181 171 136 134 125 103 76 75 71 59 54 49 48 36 7 4 AlwI GGATCnnnn'n_ Cuts at: 68 487 2201 2217 2323 2600 2610 2664 3345 Size: 419 1714 16 106 277 10 54 681 Cuts at: 3345 3358 68 Size: 13 1066 Fragments arranged by size: 1714 1066 681 419 277 106 54 16 13 10 Alw21I G_wGCw'C Cuts at: 316 2125 316 Size: 1809 2547 Alw44I G'TGCA_C Cuts at: 312 312 Size: 4356 AlwNI CAG_nnn'CTG Cuts at: 217 2567 217 Size: 2350 2006 ApaI G_GGCC'C Cuts at: 4025 4025 Size: 4356 ApaBI GCA_nnnnn'TGC Cuts at: 2568 2568 Size: 4356 ApoI r'AATT_y Cuts at: 629 640 1434 2742 3270 3726 629 Size: 11 794 1308 528 456 1259 Fragments arranged by size: 1308 1259 794 528 456 11 AvaI C'yCGr_G Cuts at: 716 1288 2218 2596 716 Size: 572 930 378 2476 Fragments arranged by size: 2476 930 572 378 AvaII G'GwC_C Cuts at: 3393 4013 4132 3393 Size: 620 119 3617 Fragments arranged by size: 3617 620 119 AvrII C'CTAG_G Cuts at: 3425 4331 3425 Size: 906 3450 BaeI ACnnnnGTAyC Cuts at: 3416 3416 Size: 4356 BamHI G'GATC_C Cuts at: 3350 3350 Size: 4356 BanI G'GyrC_C Cuts at: 867 2022 2502 3411 3442 867 Size: 1155 480 909 31 1781 Fragments arranged by size: 1781 1155 909 480 31 BanII G_rGCy'C Cuts at: 323 905 2125 4025 323 Size: 582 1220 1900 654 Fragments arranged by size: 1900 1220 654 582 BbsI GAAGACnn'nnnn_ Cuts at: 2956 2956 Size: 4356 BbvI GCAGCnnnnnnnn'nnnn_ Cuts at: 204 207 297 996 1494 2326 2422 2425 2439 Size: 3 90 699 498 832 96 3 14 Cuts at: 2439 2554 2563 3041 3244 3623 3857 4354 204 Size: 115 9 478 203 379 234 497 206 Fragments arranged by size: 832 699 498 497 478 379 234 206 203 115 96 90 14 9 3 3 BccI CCATC Cuts at: 817 837 1362 2881 3756 4196 817 Size: 20 525 1519 875 440 977 Fragments arranged by size: 1519 977 875 525 440 20 Bce83I CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 243 541 243 Size: 298 4058 BcefI ACGGCnnnnnnnnnnnn'n_ Cuts at: 127 856 1307 3838 127 Size: 729 451 2531 645 Fragments arranged by size: 2531 729 645 451 BclI T'GATC_A Cuts at: 3739 3739 Size: 4356 BfaI C'TA_G Cuts at: 133 588 595 599 981 1557 2136 2202 2651 Size: 455 7 4 382 576 579 66 449 Cuts at: 2651 2884 3109 3305 3426 3549 3696 3881 3946 Size: 233 225 196 121 123 147 185 65 Cuts at: 3946 4332 133 Size: 386 157 Fragments arranged by size: 579 576 455 449 386 382 233 225 196 185 157 147 123 121 66 65 7 4 BglI GCCn_nnn'nGGC Cuts at: 1647 1769 1840 2480 4284 1647 Size: 122 71 640 1804 1719 Fragments arranged by size: 1804 1719 640 122 71 BmgI GkGCCC Cuts at: 4023 4023 Size: 4356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 3609 3878 4055 3609 Size: 269 177 3910 Fragments arranged by size: 3910 269 177 Bpu10I CC'TnA_GC Cuts at: 1487 1487 Size: 4356 BsaI GGTCTCn'nnnn_ Cuts at: 1073 2183 1073 Size: 1110 3246 BsaAI yAC'GTr Cuts at: 830 1897 830 Size: 1067 3289 BsaBI GATnn'nnATC Cuts at: 3118 3118 Size: 4356 BsaHI Gr'CG_yC Cuts at: 1679 1732 1815 2001 1679 Size: 53 83 186 4034 Fragments arranged by size: 4034 186 83 53 BsaJI C'CnnG_G Cuts at: 466 1179 1275 1917 2341 2956 3358 3425 3939 Size: 713 96 642 424 615 402 67 514 Cuts at: 3939 4009 4017 4018 4238 4273 4282 4331 466 Size: 70 8 1 220 35 9 49 491 Fragments arranged by size: 713 642 615 514 491 424 402 220 96 70 67 49 35 9 8 1 BsaWI w'CCGG_w Cuts at: 273 420 273 Size: 147 4209 BsaXI ACnnnnnCTCC Cuts at: 775 2830 775 Size: 2055 2301 BsbI CAACAC Cuts at: 734 1405 3772 734 Size: 671 2367 1318 Fragments arranged by size: 2367 1318 671 BscGI CCCGT Cuts at: 303 924 1275 1524 1582 1974 2037 3654 4175 Size: 621 351 249 58 392 63 1617 521 Cuts at: 4175 303 Size: 484 Fragments arranged by size: 1617 621 521 484 392 351 249 63 58 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 2914 3517 4327 2914 Size: 603 810 2943 Fragments arranged by size: 2943 810 603 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 2429 3040 2429 Size: 611 3745 BsiI C'TCGT_G Cuts at: 453 453 Size: 4356 BsiEI CG_ry'CG Cuts at: 292 2643 292 Size: 2351 2005 BslI CCnn_nnn'nnGG Cuts at: 148 427 722 723 1052 1140 1248 1280 1281 Size: 279 295 1 329 88 108 32 1 Cuts at: 1281 1606 2808 3687 3897 3898 4239 148 Size: 325 1202 879 210 1 341 265 Fragments arranged by size: 1202 879 341 329 325 295 279 265 210 108 88 32 1 1 1 BsmI GAATG_Cn' Cuts at: 2843 3207 3300 2843 Size: 364 93 3899 Fragments arranged by size: 3899 364 93 BsmAI GTCTCn'nnnn_ Cuts at: 1073 1991 2183 3562 1073 Size: 918 192 1379 1867 Fragments arranged by size: 1867 1379 918 192 BsmFI GGGACnnnnnnnnnn'nnnn_ Cuts at: 1732 1883 2051 3406 3636 4026 1732 Size: 151 168 1355 230 390 2062 Fragments arranged by size: 2062 1355 390 230 168 151 BsoFI GC'n_GC Cuts at: 12 218 221 286 429 820 1010 1032 1046 Size: 206 3 65 143 391 190 22 14 Cuts at: 1046 1210 1294 1508 2315 2411 2414 2453 2552 Size: 164 84 214 807 96 3 39 99 Cuts at: 2552 2568 2640 2643 3055 3233 3612 3711 3846 Size: 16 72 3 412 178 379 99 135 Cuts at: 3846 4279 12 Size: 433 89 Fragments arranged by size: 807 433 412 391 379 214 206 190 178 164 143 135 99 99 96 89 84 72 65 39 22 16 14 3 3 3 Bsp24I GACnnnnnnTGGnnnnnnn_nnnnn' Cuts at: 110 142 1814 1846 2506 2538 110 Size: 32 1672 32 660 32 1928 Fragments arranged by size: 1928 1672 660 32 32 32 Bsp1286I G_dGCh'C Cuts at: 316 323 905 2125 4025 316 Size: 7 582 1220 1900 647 Fragments arranged by size: 1900 1220 647 582 7 BspGI CTGGAC Cuts at: 2345 4135 2345 Size: 1790 2566 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2623 2623 Size: 4356 BsrI ACTG_Gn' Cuts at: 99 211 224 740 1259 1852 2159 2394 2432 Size: 112 13 516 519 593 307 235 38 Cuts at: 2432 2469 2527 2618 4216 99 Size: 37 58 91 1598 239 Fragments arranged by size: 1598 593 519 516 307 239 235 112 91 58 38 37 13 BsrBI GAG'CGG Cuts at: 974 1115 1293 974 Size: 141 178 4037 Fragments arranged by size: 4037 178 141 BsrFI r'CCGG_y Cuts at: 931 2336 2416 931 Size: 1405 80 2871 Fragments arranged by size: 2871 1405 80 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2589 2233 Size: 356 4000 BstYI r'GATC_y Cuts at: 2206 2222 2592 2602 2656 3350 2206 Size: 16 370 10 54 694 3212 Fragments arranged by size: 3212 694 370 54 16 10 Cac8I GCn'nGC Cuts at: 16 480 576 585 589 933 976 990 1168 Size: 464 96 9 4 344 43 14 178 Cuts at: 1168 1256 1643 1836 2235 2338 2418 2448 2481 Size: 88 387 193 399 103 80 30 33 Cuts at: 2481 2563 2572 2583 2630 3023 3850 4025 16 Size: 82 9 11 47 393 827 175 347 Fragments arranged by size: 827 464 399 393 387 347 344 193 178 175 103 96 88 82 80 47 43 33 30 14 11 9 9 4 CjeI CCAnnnnnnGTnnnnnnnnn'nnnnnn_ Cuts at: 23 26 56 59 104 137 829 862 1148 Size: 3 30 3 45 33 692 33 286 Cuts at: 1148 1181 1257 1290 1615 1648 1808 1841 2367 Size: 33 76 33 325 33 160 33 526 Cuts at: 2367 2400 2500 2533 3881 3914 4094 4127 4287 Size: 33 100 33 1348 33 180 33 160 Cuts at: 4287 4320 23 Size: 33 59 Fragments arranged by size: 1348 692 526 325 286 180 160 160 100 76 59 45 33 33 33 33 33 33 33 33 33 33 33 30 3 3 CjePI GAnnnnnnnTGGnnnnnnn'nnnnnn_ Cuts at: 72 104 105 137 1808 1841 2500 2533 2811 Size: 32 1 32 1671 33 659 33 278 Cuts at: 2811 2844 2942 2975 3883 3916 72 Size: 33 98 33 908 33 512 Fragments arranged by size: 1671 908 659 512 278 98 33 33 33 33 33 32 32 1 CviJI rG'Cy Cuts at: 69 98 141 152 217 296 321 326 462 Size: 29 43 11 65 79 25 5 136 Cuts at: 462 560 587 591 598 657 677 715 819 Size: 98 27 4 7 59 20 38 104 Cuts at: 819 903 917 931 1121 1133 1170 1258 1296 Size: 84 14 14 190 12 37 88 38 Cuts at: 1296 1442 1458 1526 1599 1641 1650 1834 2123 Size: 146 16 68 73 42 9 184 289 Cuts at: 2123 2131 2158 2198 2317 2340 2416 2468 2474 Size: 8 27 40 119 23 76 52 6 Cuts at: 2474 2512 2554 2642 2725 2898 3054 3125 3235 Size: 38 42 88 83 173 156 71 110 Cuts at: 3235 3465 3473 3554 3590 3620 3638 3674 3713 Size: 230 8 81 36 30 18 36 39 Cuts at: 3713 3745 3765 3848 3852 3930 3944 3982 4008 Size: 32 20 83 4 78 14 38 26 Cuts at: 4008 4023 4064 4120 4243 4272 4278 4287 4295 Size: 15 41 56 123 29 6 9 8 Cuts at: 4295 4318 4330 4336 4349 69 Size: 23 12 6 13 76 Fragments arranged by size: 289 230 190 184 173 156 146 136 123 119 110 104 98 88 88 84 83 83 81 79 78 76 76 73 71 68 65 59 56 52 43 42 42 41 40 39 38 38 38 38 37 36 36 32 30 29 29 27 27 26 25 23 23 20 20 18 16 15 14 14 14 13 12 12 11 9 9 8 8 8 7 6 6 6 5 4 4 CviRI TG'CA Cuts at: 18 314 1473 2314 2410 2632 3057 3205 3232 Size: 296 1159 841 96 222 425 148 27 Cuts at: 3232 3300 3459 3914 4266 4342 18 Size: 68 159 455 352 76 32 Fragments arranged by size: 1159 841 455 425 352 296 222 159 148 96 76 68 32 27 DdeI C'TnA_G Cuts at: 352 1416 1487 2728 2867 4291 352 Size: 1064 71 1241 139 1424 417 Fragments arranged by size: 1424 1241 1064 417 139 71 DpnI GA'TC Cuts at: 62 494 2208 2224 2330 2539 2594 2604 2658 Size: 432 1714 16 106 209 55 10 54 Cuts at: 2658 3115 3352 3604 3741 62 Size: 457 237 252 137 677 Fragments arranged by size: 1714 677 457 432 252 237 209 137 106 55 54 16 10 DraI TTT'AAA Cuts at: 2721 3158 2721 Size: 437 3919 DraIII CAC_nnn'GTG Cuts at: 830 830 Size: 4356 DrdI GACnn_nn'nnGTC Cuts at: 524 782 524 Size: 258 4098 DrdII GAACCA Cuts at: 835 835 Size: 4356 DsaI C'CryG_G Cuts at: 1179 1275 1917 3358 4238 1179 Size: 96 642 1441 880 1297 Fragments arranged by size: 1441 1297 880 642 96 EaeI y'GGCC_r Cuts at: 817 1294 2466 2640 3850 817 Size: 477 1172 174 1210 1323 Fragments arranged by size: 1323 1210 1172 477 174 EagI C'GGCC_G Cuts at: 2640 2640 Size: 4356 EarI CTCTTCn'nnn_ Cuts at: 2931 3403 4040 2931 Size: 472 637 3247 Fragments arranged by size: 3247 637 472 EciI TCCGCC Cuts at: 410 556 2065 2394 2484 4192 4213 4223 4234 Size: 146 1509 329 90 1708 21 10 11 Cuts at: 4234 410 Size: 532 Fragments arranged by size: 1708 1509 532 329 146 90 21 11 10 Eco57I CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 84 2419 2540 2953 3654 3731 84 Size: 2335 121 413 701 77 709 Fragments arranged by size: 2335 709 701 413 121 77 EcoNI CCTnn'n_nnAGG Cuts at: 3685 3685 Size: 4356 EcoO109I rG'GnC_Cy Cuts at: 3393 3763 4013 3393 Size: 370 250 3736 Fragments arranged by size: 3736 370 250 EcoRII 'CCwGG_ Cuts at: 465 1645 1838 2341 3939 4017 465 Size: 1180 193 503 1598 78 804 Fragments arranged by size: 1598 1180 804 503 193 78 FauI CCCGCnnnn'nn_ Cuts at: 967 1036 1108 1243 1650 1676 1843 2439 2556 Size: 69 72 135 407 26 167 596 117 Cuts at: 2556 3362 3484 4032 4172 4207 967 Size: 806 122 548 140 35 1116 Fragments arranged by size: 1116 806 596 548 407 167 140 135 122 117 72 69 35 26 FokI GGATGnnnnnnnnn'nnnn_ Cuts at: 3847 4182 3847 Size: 335 4021 GdiII y'GGCC_G Cuts at: 817 1294 2466 2640 3850 817 Size: 477 1172 174 1210 1323 Fragments arranged by size: 1323 1210 1172 477 174 HaeI wGG'CCw Cuts at: 152 1258 3944 4330 152 Size: 1106 2686 386 178 Fragments arranged by size: 2686 1106 386 178 HaeII r_GCGC'y Cuts at: 386 981 989 2423 2453 2529 2568 386 Size: 595 8 1434 30 76 39 2174 Fragments arranged by size: 2174 1434 595 76 39 30 8 HaeIII GG'CC Cuts at: 152 677 819 1133 1258 1296 1526 1641 1834 Size: 525 142 314 125 38 230 115 193 Cuts at: 1834 2340 2468 2642 3765 3852 3944 4008 4023 Size: 506 128 174 1123 87 92 64 15 Cuts at: 4023 4120 4272 4278 4287 4330 152 Size: 97 152 6 9 43 178 Fragments arranged by size: 1123 525 506 314 230 193 178 174 152 142 128 125 115 97 92 87 64 43 38 15 9 6 HgaI GACGCnnnnn'nnnnn_ Cuts at: 514 1203 2083 2468 3851 514 Size: 689 880 385 1383 1019 Fragments arranged by size: 1383 1019 880 689 385 HgiEII ACCnnnnnnGGT Cuts at: 44 44 Size: 4356 HhaI G_CG'C Cuts at: 111 285 385 452 980 988 1014 1036 1045 Size: 174 100 67 528 8 26 22 9 Cuts at: 1045 1058 1214 1233 1427 1507 2239 2422 2452 Size: 13 156 19 194 80 732 183 30 Cuts at: 2452 2528 2567 2628 111 Size: 76 39 61 1839 Fragments arranged by size: 1839 732 528 194 183 174 156 100 80 76 67 61 39 30 26 22 19 13 9 8 Hin4I GAbnnnnnvTC Cuts at: 1313 2246 2292 2525 2542 3111 3620 4279 1313 Size: 933 46 233 17 569 509 659 1390 Fragments arranged by size: 1390 933 659 569 509 233 46 17 HincII GTy'rAC Cuts at: 1541 3219 1541 Size: 1678 2678 HindIII A'AGCT_T Cuts at: 2196 2196 Size: 4356 HinfI G'AnT_C Cuts at: 256 754 776 1107 1309 1344 1384 1480 1967 Size: 498 22 331 202 35 40 96 487 Cuts at: 1967 2176 2242 2488 2544 2647 2774 2798 2907 Size: 209 66 246 56 103 127 24 109 Cuts at: 2907 2990 3649 3791 256 Size: 83 659 142 821 Fragments arranged by size: 821 659 498 487 331 246 209 202 142 127 109 103 96 83 66 56 40 35 24 22 HpaI GTT'AAC Cuts at: 3219 3219 Size: 4356 HphI GGTGAnnnnnnn_n' Cuts at: 831 1933 831 Size: 1102 3254 KpnI G_GTAC'C Cuts at: 3415 3415 Size: 4356 MaeII A'CG_T Cuts at: 612 771 783 829 939 1090 1194 1679 1691 Size: 159 12 46 110 151 104 485 12 Cuts at: 1691 1732 1815 1896 2001 612 Size: 41 83 81 105 2967 Fragments arranged by size: 2967 485 159 151 110 105 104 83 81 46 41 12 12 MaeIII 'GTnAC_ Cuts at: 90 206 269 1003 1015 1195 1618 1705 2054 Size: 116 63 734 12 180 423 87 349 Cuts at: 2054 2687 3244 4106 4124 90 Size: 633 557 862 18 322 Fragments arranged by size: 862 734 633 557 423 349 322 180 116 87 63 18 12 MboII GAAGAnnnnnnn_n' Cuts at: 970 1489 2597 2883 2948 2961 3390 3763 4057 Size: 519 1108 286 65 13 429 373 294 Cuts at: 4057 970 Size: 1269 Fragments arranged by size: 1269 1108 519 429 373 294 286 65 13 MluI A'CGCG_T Cuts at: 1535 1535 Size: 4356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 233 417 804 1084 1386 2826 3793 233 Size: 184 387 280 302 1440 967 796 Fragments arranged by size: 1440 967 796 387 302 280 184 MnlI CCTCnnnnnn_n' Cuts at: 199 449 523 858 1109 1123 1402 2098 2221 Size: 250 74 335 251 14 279 696 123 Cuts at: 2221 2227 2350 2592 2647 2843 2888 2935 3135 Size: 6 123 242 55 196 45 47 200 Cuts at: 3135 3175 3184 3406 3433 3535 3538 3609 3681 Size: 40 9 222 27 102 3 71 72 Cuts at: 3681 3691 3754 3776 3795 3818 3874 3891 3902 Size: 10 63 22 19 23 56 17 11 Cuts at: 3902 3911 3941 3998 4043 4054 4064 4151 4262 Size: 9 30 57 45 11 10 87 111 Cuts at: 4262 4268 4292 4298 4305 4308 4320 199 Size: 6 24 6 7 3 12 235 Fragments arranged by size: 696 335 279 251 250 242 235 222 200 196 123 123 111 102 87 74 72 71 63 57 56 55 47 45 45 40 30 27 24 23 22 19 17 14 12 11 11 10 10 9 9 7 6 6 6 3 3 MseI T'TA_A Cuts at: 615 626 638 649 666 764 1038 1138 1438 Size: 11 12 11 17 98 274 100 300 Cuts at: 1438 1564 2169 2720 2748 2764 2845 3157 3218 Size: 126 605 551 28 16 81 312 61 Cuts at: 3218 3796 3820 3870 3998 4155 615 Size: 578 24 50 128 157 816 Fragments arranged by size: 816 605 578 551 312 300 274 157 128 126 100 98 81 61 50 28 24 17 16 12 11 11 MslI CAynn'nnrTG Cuts at: 1922 3970 1922 Size: 2048 2308 MspI C'CG_G Cuts at: 58 248 274 421 932 2337 2417 4009 58 Size: 190 26 147 511 1405 80 1592 405 Fragments arranged by size: 1592 1405 511 405 190 147 80 26 MspA1I CmG'CkG Cuts at: 43 288 1170 1181 1209 3417 3479 3848 3982 Size: 245 882 11 28 2208 62 369 134 Cuts at: 3982 43 Size: 417 Fragments arranged by size: 2208 882 417 369 245 134 62 28 11 MunI C'AATT_G Cuts at: 1468 3206 1468 Size: 1738 2618 MwoI GCnn_nnn'nnGC Cuts at: 8 580 584 819 941 971 998 1040 1042 Size: 572 4 235 122 30 27 42 2 Cuts at: 1042 1504 1647 1769 1801 1840 1933 1957 2419 Size: 462 143 122 32 39 93 24 462 Cuts at: 2419 2428 2452 2480 2567 2571 3027 3617 3854 Size: 9 24 28 87 4 456 590 237 Cuts at: 3854 4199 4278 4284 4355 8 Size: 345 79 6 71 9 Fragments arranged by size: 590 572 462 462 456 345 237 235 143 122 122 93 87 79 71 42 39 32 30 28 27 24 24 9 9 6 4 4 2 NciI CC's_GG Cuts at: 249 4010 249 Size: 3761 595 NcoI C'CATG_G Cuts at: 1917 3358 4238 1917 Size: 1441 880 2035 Fragments arranged by size: 2035 1441 880 NdeI CA'TA_TG Cuts at: 1791 1791 Size: 4356 NgoAIV G'CCGG_C Cuts at: 931 2336 2416 931 Size: 1405 80 2871 Fragments arranged by size: 2871 1405 80 NheI G'CTAG_C Cuts at: 587 587 Size: 4356 NlaIII _CATG' Cuts at: 1477 1861 1921 2305 2479 2997 3346 3362 4242 Size: 384 60 384 174 518 349 16 880 Cuts at: 4242 1477 Size: 1591 Fragments arranged by size: 1591 880 518 384 384 349 174 60 16 NlaIV GGn'nCC Cuts at: 559 869 890 902 1286 2024 2504 2671 3352 Size: 310 21 12 384 738 480 167 681 Cuts at: 3352 3394 3413 3444 3714 4014 4015 4023 559 Size: 42 19 31 270 300 1 8 892 Fragments arranged by size: 892 738 681 480 384 310 300 270 167 42 31 21 19 12 8 1 NotI GC'GGCC_GC Cuts at: 2640 2640 Size: 4356 NruI TCG'CGA Cuts at: 1515 3601 1515 Size: 2086 2270 NspV TT'CG_AA Cuts at: 1334 1342 1334 Size: 8 4348 PflMI CCAn_nnn'nTGG Cuts at: 2808 2808 Size: 4356 PleI GAGTCnnnn'n_ Cuts at: 250 762 770 1303 1961 2170 2641 2901 2998 Size: 512 8 533 658 209 471 260 97 Cuts at: 2998 3657 3785 250 Size: 659 128 821 Fragments arranged by size: 821 659 658 533 512 471 260 209 128 97 8 Psp5II rG'GwC_Cy Cuts at: 3393 4013 3393 Size: 620 3736 Psp1406I AA'CG_TT Cuts at: 612 612 Size: 4356 PstI C_TGCA'G Cuts at: 2634 2634 Size: 4356 PvuII CAG'CTG Cuts at: 1170 3848 3982 1170 Size: 2678 134 1544 Fragments arranged by size: 2678 1544 134 RleAI CCCACAnnnnnnnnn_nnn' Cuts at: 3185 3185 Size: 4356 RsaI GT'AC Cuts at: 1061 1163 1521 1776 1801 1856 1889 1940 2097 Size: 102 358 255 25 55 33 51 157 Cuts at: 2097 3413 3507 1061 Size: 1316 94 1910 Fragments arranged by size: 1910 1316 358 255 157 102 94 55 51 33 25 SacI G_AGCT'C Cuts at: 2125 2125 Size: 4356 SacII CC_GC'GG Cuts at: 1182 1182 Size: 4356 Sau96I G'GnC_C Cuts at: 1524 1640 1833 2339 3393 3763 4013 4021 4022 Size: 116 193 506 1054 370 250 8 1 Cuts at: 4022 4119 4132 1524 Size: 97 13 1748 Fragments arranged by size: 1748 1054 506 370 250 193 116 97 13 8 1 Sau3AI 'GATC_ Cuts at: 60 492 2206 2222 2328 2537 2592 2602 2656 Size: 432 1714 16 106 209 55 10 54 Cuts at: 2656 3113 3350 3602 3739 60 Size: 457 237 252 137 677 Fragments arranged by size: 1714 677 457 432 252 237 209 137 106 55 54 16 10 ScaI AGT'ACT Cuts at: 3507 3507 Size: 4356 ScrFI CC'n_GG Cuts at: 249 467 1647 1840 2343 3941 4010 4019 249 Size: 218 1180 193 503 1598 69 9 586 Fragments arranged by size: 1598 1180 586 503 218 193 69 9 SfaNI GCATCnnnnn'nnnn_ Cuts at: 529 1218 1914 3272 3468 529 Size: 689 696 1358 196 1417 Fragments arranged by size: 1417 1358 696 689 196 SfcI C'TryA_G Cuts at: 170 361 1049 2182 2630 2710 170 Size: 191 688 1133 448 80 1816 Fragments arranged by size: 1816 1133 688 448 191 80 SfiI GGCCn_nnn'nGGCC Cuts at: 4284 4284 Size: 4356 SnaBI TAC'GTA Cuts at: 1897 1897 Size: 4356 SpeI A'CTAG_T Cuts at: 1556 1556 Size: 4356 Sse8387I CC_TGCA'GG Cuts at: 2634 2634 Size: 4356 SspI AAT'ATT Cuts at: 619 3087 619 Size: 2468 1888 StuI AGG'CCT Cuts at: 3944 4330 3944 Size: 386 3970 StyI C'CwwG_G Cuts at: 1917 2956 3358 3425 4238 4331 1917 Size: 1039 402 67 813 93 1942 Fragments arranged by size: 1942 1039 813 402 93 67 TaqI T'CG_A Cuts at: 528 863 1328 1334 1342 2163 2213 2219 2457 Size: 335 465 6 8 821 50 6 238 Cuts at: 2457 2597 3484 3683 528 Size: 140 887 199 1201 Fragments arranged by size: 1201 887 821 465 335 238 199 140 50 8 6 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 856 1478 1729 3953 3974 856 Size: 622 251 2224 21 1238 Fragments arranged by size: 2224 1238 622 251 21 TfiI G'AwT_C Cuts at: 1107 1344 1384 1480 2242 2488 2544 2774 2798 Size: 237 40 96 762 246 56 230 24 Cuts at: 2798 1107 Size: 2665 Fragments arranged by size: 2665 762 246 237 230 96 56 40 24 ThaI CG'CG Cuts at: 635 1014 1034 1058 1181 1214 1427 1515 1537 Size: 379 20 24 123 33 213 88 22 Cuts at: 1537 1617 2239 2639 2645 3601 635 Size: 80 622 400 6 956 1390 Fragments arranged by size: 1390 956 622 400 379 213 123 88 80 33 24 22 20 6 TseI GCwGC Cuts at: 12 218 221 286 1010 1508 2315 2411 2414 Size: 206 3 65 724 498 807 96 3 Cuts at: 2414 2453 2552 2568 3055 3233 3612 3846 12 Size: 39 99 16 487 178 379 234 522 Fragments arranged by size: 807 724 522 498 487 379 234 206 178 99 96 65 39 16 3 3 Tsp45I 'GTsAC_ Cuts at: 1003 2687 4106 4124 1003 Size: 1684 1419 18 1235 Fragments arranged by size: 1684 1419 1235 18 Tsp509I 'AATT_ Cuts at: 603 629 640 1434 1468 1575 2166 2694 2742 Size: 26 11 794 34 107 591 528 48 Cuts at: 2742 2779 2973 3073 3206 3270 3594 3726 3778 Size: 37 194 100 133 64 324 132 52 Cuts at: 3778 3871 4249 4351 603 Size: 93 378 102 608 Fragments arranged by size: 794 608 591 528 378 324 194 133 132 107 102 100 93 64 52 48 37 34 26 11 Tth111II CAArCAnnnnnnnnn_nn' Cuts at: 3 35 42 1386 2314 3010 3508 3 Size: 32 7 1344 928 696 498 851 Fragments arranged by size: 1344 928 851 696 498 32 7 UbaJI GCsGC Cuts at: 429 820 1032 1046 1210 1294 2640 2643 3711 Size: 391 212 14 164 84 1346 3 1068 Cuts at: 3711 4279 429 Size: 568 506 Fragments arranged by size: 1346 1068 568 506 391 212 164 84 14 3 VspI AT'TA_AT Cuts at: 1564 2169 1564 Size: 605 3751 XbaI T'CTAG_A Cuts at: 2201 2650 2201 Size: 449 3907 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3902 3902 Size: 4356 XhoI C'TCGA_G Cuts at: 2218 2596 2218 Size: 378 3978 Enzymes that do cut: AatII AccI AceIII AciI AflIII AluI AlwI Alw21I Alw44I AlwNI ApaI ApaBI ApoI AvaI AvaII AvrII BaeI BamHI BanI BanII BbsI BbvI BccI Bce83I BcefI BclI BfaI BglI BmgI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaJI BsaWI BsaXI BsbI BscGI BseRI BsgI BsiI BsiEI BslI BsmI BsmAI BsmFI BsoFI Bsp24I Bsp1286I BspGI BspMI BsrI BsrBI BsrFI BstXI BstYI Cac8I CjeI CjePI CviJI CviRI DdeI DpnI DraI DraIII DrdI DrdII DsaI EaeI EagI EarI EciI Eco57I EcoNI EcoO109I EcoRII FauI FokI GdiII HaeI HaeII HaeIII HgaI HgiEII HhaI Hin4I HincII HindIII HinfI HpaI HphI KpnI MaeII MaeIII MboII MluI MmeI MnlI MseI MslI MspI MspA1I MunI MwoI NciI NcoI NdeI NgoAIV NheI NlaIII NlaIV NotI NruI NspV PflMI PleI Psp5II Psp1406I PstI PvuII RleAI RsaI SacI SacII Sau96I Sau3AI ScaI ScrFI SfaNI SfcI SfiI SnaBI SpeI Sse8387I SspI StuI StyI TaqI TaqII TfiI ThaI TseI Tsp45I Tsp509I Tth111II UbaJI VspI XbaI XcmI XhoI Enzymes that do not cut: AflII AgeI AscI BcgI BcgI BglII Bpu1102I BsmBI BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstEII Bsu36I ClaI Eam1105I Eco47III EcoRI EcoRV FseI FspI MscI NarI NsiI NspI PacI Pfl1108I PmeI PmlI PshAI PvuI RcaI RsrII SalI SapI SexAI SgfI SgrAI SmaI SphI SrfI SunI SwaI Tth111I XmnI