General Description
DNA pCR2_1 VPCP
Entire molecule length: 5504 bp
Restriction Map
Enzyme |
# of cuts |
Positions |
Acc65I |
1 |
241 |
AciI |
61 |
19 29 85(c) 188(c) 270 298 308 1223 1332(c) 1515(c)
1582(c) 1628(c) 1639(c) 1649(c) 1661(c) 1670(c) 1682(c) 1924(c) 1928 2095
2157(c) 2171(c) 2174(c) 2202 2229 2344 2483(c) 2608 2731(c) 2827 2930 2993
3087(c) 3151(c) 3252(c) 3255(c) 3495 3535(c) 3540 3590(c) 3606 3632 3747(c)
3856(c) 3983(c) 4098(c) 4144 4335(c) 4426(c) 4788 4797(c) 4932 5042(c)
5163(c) 5182(c) 5309(c) 5337(c) 5428 5449 5456 5499(c) |
AcsI |
5 |
284 465 1244 1898 2556 |
AcyI |
3 |
2773 3021 3952 |
AflIII |
3 |
1381 2591 5380 |
AluI |
29 |
56 120 215 237 249 504 929 1017 1068 1450 1524 1568
2073 2288 2327 2545 2642 2695 2721 2767 3127 3585 4139 4202 4302 4823 5080
5216 5442 |
Alw44I |
2 |
3820 5066 |
AlwI |
20 |
248(c) 261 380(c) 1213(c) 1226 2842 2859 2889 3200
3265(c) 3444(c) 3446 3853 3857(c) 4174 4637(c) 4638 4734(c) 4736 4822 |
AlwNI |
2 |
2645 4971 |
AosI |
3 |
2123 3123 4269 |
ApaI |
2 |
1507 1953 |
ApaLI |
2 |
3820 5066 |
ApoI |
5 |
284 465 1244 1898 2556 |
AseI |
5 |
48 107 327 356 4317 |
AsnI |
5 |
48 107 327 356 4317 |
Asp700 |
1 |
3892 |
Asp718 |
1 |
241 |
AspEI |
1 |
4492 |
AspHI |
8 |
251 866 931 3134 3324 3824 3909 5070 |
AspI |
1 |
3139 |
AvaI |
2 |
1359 1931 |
AvaII |
4 |
1356 3537 4128 4350 |
AviII |
3 |
2123 3123 4269 |
BamHI |
2 |
253 1218 |
BanI |
9 |
132 241 752 941 2334 2772 3020 3055 4539 |
BanII |
6 |
251 931 1507 1953 2304 3386 |
BbvI |
21 |
65 491(c) 797(c) 2063 2136 2205 2560(c) 2968(c)
3094 3136 3152(c) 3245(c) 3657 4058(c) 4449 4752(c) 4958(c) 4961(c) 5051 5470
5488 |
BcgI |
1 |
3954(c) |
BclI |
2 |
622 2862 |
BfaI |
15 |
260 432 636 709 889 1014 1018 1402 1521 1944 2222
2722 4299 4634 4887 |
BglI |
3 |
1582 2133 4374 |
BglII |
2 |
1528 2857 |
BmyI |
15 |
251 755 866 931 1507 1953 2304 2967 3060 3134 3324
3386 3824 3909 5070 |
BpmI |
1 |
4423 |
BsaAI |
3 |
2375 2594 3325 |
BsaBI |
5 |
621 1394 1912 2856 2880 |
BsaHI |
3 |
2773 3021 3952 |
BsaI |
2 |
370(c) 4426 |
BsaJI |
22 |
137 759 1123 1140 1191 1226 1269 1359 1360 1458
1507 1577 1586 1621 1744 1808 2023 2626 2830 3184 3453 5220 |
BsaWI |
6 |
2585 2754 3052 4196 5027 5174 |
BsgI |
1 |
1079(c) |
BsiEI |
10 |
270 1474 1928 2104 2344 2930 3974 4123 5046 5470 |
BsiHKAI |
8 |
251 866 931 3134 3324 3824 3909 5070 |
BsiYI |
14 |
35 314 994 1359 1360 1627 2156 2482 3002 3546 4902
5181 5347 5365 |
BslI |
14 |
35 314 994 1359 1360 1627 2156 2482 3002 3546 4902
5181 5347 5365 |
BsmAI |
3 |
370(c) 2863(c) 4426 |
BsmFI |
7 |
1358(c) 1439(c) 1698 1762 1826 2156 3172 |
BsmI |
2 |
441 534(c) |
Bsp120I |
2 |
1503 1949 |
Bsp1286I |
15 |
251 755 866 931 1507 1953 2304 2967 3060 3134 3324
3386 3824 3909 5070 |
BspHI |
1 |
4660 |
BspMI |
4 |
323(c) 2679(c) 2908(c) 3289 |
BspWI |
34 |
11 95 139 963 1521 1582 1588 1667 1718 1782 1902
1933 1957 2103 2133 2166 2168 2210 2237 2267 2573 2712 3012 3096 3119 3258
3264 3381 3417 3464 4374 4762 5334 5448 |
BsrBI |
5 |
188 1930(c) 2231(c) 3634(c) 5451(c) |
BsrDI |
3 |
3254 4258 4432(c) |
BsrFI |
5 |
379 2270 3340 3521 4407 |
BsrI |
23 |
82 273(c) 728(c) 1075(c) 1649 1925 1992 2018 2464
2603(c) 2650 2731 2815 2965 3166 3847 4017(c) 4286 4329 4447 4853 4965(c)
4978(c) |
BssHII |
1 |
3418 |
BstNI |
13 |
139 342 760 1028 1192 1460 1745 1809 2025 3408 5221
5234 5355 |
BstUI |
16 |
31 33 310 312 1407 2147 2171 2191 2483 3087 3388
3420 3983 4426 4756 5337 |
BstXI |
2 |
280 1920 |
BstYI |
14 |
253 385 1218 1528 2834 2857 3192 3438 3845 3862
4630 4642 4728 4739 |
CfoI |
36 |
5 33 98 312 1322 1407 1419 1520 2124 2149 2162 2171
2193 2219 2227 2567 2677 2775 2846 3015 3023 3087 3124 3390 3420 3422 3650
3983 4270 4363 4756 4865 5039 5139 5206 5476 |
Cfr10I |
5 |
379 2270 3340 3521 4407 |
Csp6I |
7 |
242 701 920 1156 1256 3326 4010 |
DdeI |
9 |
909 925 971 1525 1569 3991 4531 4697 5106 |
DpnI |
31 |
255 387 624 1220 1391 1530 2103 2836 2853 2859 2864
2883 3194 3272 3353 3362 3440 3451 3811 3847 3864 4122 4168 4186 4527 4632
4644 4722 4730 4741 4816 |
DpnII |
31 |
253 385 622 1218 1389 1528 2101 2834 2851 2857 2862
2881 3192 3270 3351 3360 3438 3449 3809 3845 3862 4120 4166 4184 4525 4630
4642 4720 4728 4739 4814 |
DraI |
4 |
581 3914 4606 4625 |
DraII |
3 |
763 1503 1949 |
DraIII |
3 |
912 998 2378 |
DrdI |
3 |
2422 3048 5278 |
DsaI |
6 |
1123 1140 1226 1507 1621 3453 |
DsaV |
24 |
137 340 758 994 1026 1190 1352 1358 1359 1458 1743
1807 2023 2626 3023 3183 3406 3954 3976 4305 5001 5219 5232 5353 |
EaeI |
11 |
37 267 316 1306 1925 1989 2927 3101 3492 3519 4099 |
EagI |
3 |
267 1925 2927 |
Eam1105I |
1 |
4492 |
EarI |
6 |
1553 2082(c) 3365(c) 3575(c) 3693(c) 5497(c) |
Ecl136II |
2 |
249 929 |
EclXI |
3 |
267 1925 2927 |
Eco57I |
4 |
3167 3599 3826 4838(c) |
EcoO109I |
3 |
763 1503 1949 |
EcoRI |
3 |
284 1244 1898 |
EcoRII |
13 |
137 340 758 1026 1190 1458 1743 1807 2023 3406 5219
5232 5353 |
EcoRV |
2 |
694 1910 |
EheI |
2 |
2774 3022 |
Fnu4HI |
42 |
54 270 505 811 1516 1583 1925 1928 2052 2125 2158
2172 2194 2574 2827 2930 2982 2993 3083 3088 3125 3166 3253 3256 3259 3495
3591 3632 3646 3748 4072 4099 4438 4766 4972 4975 5040 5183 5338 5456 5459
5477 |
FnuDII |
16 |
31 33 310 312 1407 2147 2171 2191 2483 3087 3388
3420 3983 4426 4756 5337 |
FokI |
13 |
711(c) 782(c) 1010(c) 1677 2043(c) 2643 2826 2888
3345 3370 4053 4340 4521 |
FspI |
3 |
2123 3123 4269 |
HaeII |
8 |
6 1323 1521 2220 2228 2776 3024 5140 |
HaeIII |
31 |
39 269 318 656 765 823 1022 1139 1145 1308 1505
1576 1585 1591 1927 1951 1991 2093 2383 2525 2929 3103 3494 3521 4101 4368
4448 4906 5340 5358 5369 |
HgaI |
5 |
1396(c) 2153 3960 4690(c) 5268(c) |
HgiAI |
8 |
251 866 931 3134 3324 3824 3909 5070 |
HhaI |
36 |
5 33 98 312 1322 1407 1419 1520 2124 2149 2162 2171
2193 2219 2227 2567 2677 2775 2846 3015 3023 3087 3124 3390 3420 3422 3650
3983 4270 4363 4756 4865 5039 5139 5206 5476 |
HinP1I |
36 |
3 31 96 310 1320 1405 1417 1518 2122 2147 2160 2169
2191 2217 2225 2565 2675 2773 2844 3013 3021 3085 3122 3388 3418 3420 3648
3981 4268 4361 4754 4863 5037 5137 5204 5474 |
HincII |
2 |
338 520 |
HindII |
2 |
338 520 |
HindIII |
1 |
235 |
HinfI |
16 |
42 321 363 740 792 1335 1365 1970 2423 2445 3506
3640 4493 5010 5406 5481 |
HpaI |
2 |
338 520 |
HpaII |
27 |
160 380 995 1354 1360 2271 2586 2628 2755 2926 3003
3025 3053 3184 3274 3341 3522 3955 3977 4197 4307 4374 4408 4812 5002 5028
5175 |
HphI |
10 |
688(c) 773(c) 778(c) 1855(c) 2375 3765(c) 3800
4006(c) 4422 4649 |
ItaI |
42 |
54 270 505 811 1516 1583 1925 1928 2052 2125 2158
2172 2194 2574 2827 2930 2982 2993 3083 3088 3125 3166 3253 3256 3259 3495
3591 3632 3646 3748 4072 4099 4438 4766 4972 4975 5040 5183 5338 5456 5459
5477 |
KasI |
2 |
2772 3020 |
KpnI |
1 |
245 |
Ksp632I |
6 |
1553 2082(c) 3365(c) 3575(c) 3693(c) 5497(c) |
MaeI |
15 |
260 432 636 709 889 1014 1018 1402 1521 1944 2222
2722 4299 4634 4887 |
MaeII |
12 |
1428 2005 2264 2374 2417 2429 2593 3137 3324 3890
4263 4679 |
MaeIII |
18 |
262 490 737 904 1861 2009 2029 2185 2197 3141 3831
4019 4172 4230 4561 4844 4960 5023 |
MamI |
5 |
621 1394 1912 2856 2880 |
MboI |
31 |
253 385 622 1218 1389 1528 2101 2834 2851 2857 2862
2881 3192 3270 3351 3360 3438 3449 3809 3845 3862 4120 4166 4184 4525 4630
4642 4720 4728 4739 4814 |
MboII |
14 |
10 652(c) 1110(c) 1540(c) 2099 2236(c) 3382 3592
3672(c) 3710 3819 3897 4652 4723(c) |
McrI |
10 |
270 1474 1928 2104 2344 2930 3974 4123 5046 5470 |
MfeI |
1 |
529 |
MluNI |
1 |
3103 |
MnlI |
35 |
32 311 556(c) 565(c) 605 674 794 879 932(c) 955
966(c) 1442 1544 1556 1566(c) 1572(c) 1596 1602 1940(c) 2083(c) 2348 2787
2872(c) 2936(c) 3072(c) 3429(c) 3622 4119(c) 4325(c) 4472 4553 4953 5203(c)
5277 5486(c) |
MscI |
1 |
3103 |
MseI |
25 |
48 107 327 337 356 519 580 715 855 882 1185 1441
2040 2165 2436 2534 2551 3913 4278 4317 4552 4605 4619 4624 4676 |
MslI |
4 |
3458 3721 4080 4239 |
MspA1I |
7 |
56 2073 2767 3127 3856 4797 5042 |
MspI |
27 |
160 380 995 1354 1360 2271 2586 2628 2755 2926 3003
3025 3053 3184 3274 3341 3522 3955 3977 4197 4307 4374 4408 4812 5002 5028
5175 |
MunI |
1 |
529 |
MvaI |
13 |
139 342 760 1028 1192 1460 1745 1809 2025 3408 5221
5234 5355 |
MvnI |
16 |
31 33 310 312 1407 2147 2171 2191 2483 3087 3388
3420 3983 4426 4756 5337 |
MwoI |
34 |
11 95 139 963 1521 1582 1588 1667 1718 1782 1902
1933 1957 2103 2133 2166 2168 2210 2237 2267 2573 2712 3012 3096 3119 3258
3264 3381 3417 3464 4374 4762 5334 5448 |
NaeI |
2 |
2272 3523 |
NarI |
2 |
2773 3021 |
NciI |
11 |
996 1354 1360 1361 2628 3025 3185 3956 3978 4307
5003 |
NcoI |
5 |
1140 1226 1507 1621 3453 |
NdeII |
31 |
253 385 622 1218 1389 1528 2101 2834 2851 2857 2862
2881 3192 3270 3351 3360 3438 3449 3809 3845 3862 4120 4166 4184 4525 4630
4642 4720 4728 4739 4814 |
NgoMI |
2 |
2270 3521 |
NheI |
1 |
1520 |
NlaIII |
27 |
226 397 839 1144 1156 1174 1230 1244 1385 1417 1511
1625 1716 1780 1940 2714 2895 3240 3426 3457 3483 4049 4085 4163 4173 4664
5384 |
NlaIV |
27 |
134 243 255 655 754 764 943 1146 1220 1358 1505
1742 1806 1951 2303 2315 2336 2584 2774 3022 3057 4195 4406 4447 4541 5313
5352 |
NotI |
1 |
1925 |
NsiI |
3 |
1714 1778 1942 |
NspI |
7 |
1385 1417 1716 1780 1940 3426 5384 |
PaeR7I |
1 |
1931 |
PleI |
10 |
357(c) 734(c) 786(c) 1343 1978 2431 2439(c) 4501
5004(c) 5489 |
Ppu10I |
3 |
1710 1774 1938 |
Psh A1 |
1 |
1470 |
Psp1406I |
2 |
3890 4263 |
PstI |
3 |
815 1907 3074 |
PvuI |
2 |
2104 4123 |
PvuII |
4 |
56 2073 2767 3127 |
RcaI |
1 |
4660 |
RsaI |
7 |
243 702 921 1157 1257 3327 4011 |
RsrII |
1 |
3537 |
SacI |
2 |
251 931 |
SapI |
3 |
3365(c) 3575(c) 5497(c) |
Sau3AI |
31 |
253 385 622 1218 1389 1528 2101 2834 2851 2857 2862
2881 3192 3270 3351 3360 3438 3449 3809 3845 3862 4120 4166 4184 4525 4630
4642 4720 4728 4739 4814 |
Sau96I |
16 |
654 763 822 1144 1356 1503 1504 1949 1950 2092 2381
3537 4128 4350 4367 4446 |
ScaI |
1 |
4011 |
ScrFI |
24 |
139 342 760 996 1028 1192 1354 1360 1361 1460 1745
1809 2025 2628 3025 3185 3408 3956 3978 4307 5003 5221 5234 5355 |
SfaNI |
17 |
463(c) 733 966 1284 1699(c) 1763(c) 1949 2979(c)
3234(c) 3320 3384 3450(c) 3659 3791(c) 4040 4231(c) 5283(c) |
SfcI |
11 |
369 598 811 1855 1903 1963 2152 3070 4246 4924 5115
|
SfiI |
1 |
1582 |
SmaI |
1 |
1361 |
SnoI |
2 |
3820 5066 |
SpeI |
1 |
259 |
SphI |
5 |
1417 1716 1780 1940 3426 |
StyI |
7 |
1140 1226 1269 1507 1621 2830 3453 |
TaqI |
13 |
350 1206 1299 1392 1848 1932 2340 3134 3290 3314
3350 3838 5282 |
TfiI |
6 |
42 321 1365 3506 3640 5406 |
ThaI |
16 |
31 33 310 312 1407 2147 2171 2191 2483 3087 3388
3420 3983 4426 4756 5337 |
Tru9I |
25 |
48 107 327 337 356 519 580 715 855 882 1185 1441
2040 2165 2436 2534 2551 3913 4278 4317 4552 4605 4619 4624 4676 |
Tsp509I |
22 |
104 179 196 284 353 465 529 712 1244 1264 1610 1702
1766 1898 1954 1981 2556 2758 3686 4059 4314 4620 |
Tth111I |
1 |
3139 |
XbaI |
2 |
635 1943 |
XcmI |
1 |
2837 |
XhoI |
1 |
1931 |
XhoII |
14 |
253 385 1218 1528 2834 2857 3192 3438 3845 3862
4630 4642 4728 4739 |
XmaI |
1 |
1359 |
XmaIII |
3 |
267 1925 2927 |
XmnI |
1 |
3892 |
cspI |
1 |
3537 |
No
cuts: AatI, AatII, AccI, AccIII, AflII, AgeI, AscI, AsuII, AvrII, BbrPI, BbsI,
BfrI, BlnI, Bpu1102I, BpuAI, BseAI, BsiWI, BsmBI, BspDI, BspEI, BsrGI,
Bst1107I, BstBI, BstEII, Bsu36I, CelII, ClaI, Csp45I, Eco47III, EcoNI, Esp3I,
EspI, Fse I, FseI, KspI, MluI, MroI, NdeI, NruI, NspV, PacI, PflMI, PinAI,
PmaCI, PmeI, PmlI, PpuMI, SacII, SalI, SexAI, SfuI, SgrAI, SnaBI, SrfI, SspBI,
SspI, StuI, SwaI, Van91I