File: pMTBiP/V5-His B 24-0429 Mode: Normal 1 - 3646 Table: DNASIS AVAIL Style: Circular Indication Mode: Actual Cutting Site Enzyme Name Sequence Count Cutting Position AatII GACGT!C 1 3583 Acc65I G!GTACC 1 923 AccI GT!MKAC 1 1392 AflII C!TTAAG 0 AflIII A!CRYGT 3 660 1047 1768 AgeI A!CCGGT 1 1053 AluI AG!CT 19 45 64 309 447 652 1125 1267 1411 1433 1528 1592 1710 1936 2026 2072 2329 2850 2950 3013 AlwI GGATCNNNN! 5 845 1156 2417 2514 3294 AlwI !NNNNNGATCC 7 832 1143 2329 2415 2513 2977 3298 AlwNI CAGNNN!CTG 1 2184 ApaI GGGCC!C 1 996 ApaLI G!TGCAC 3 178 2082 3328 ApoI R!AATTY 4 403 674 947 1228 AscI GG!CGCGCC 0 AseI AT!TAAT 3 1539 1598 2833 AvaI C!YCGRG 3 900 918 980 AvaII G!GWCC 2 2799 3021 AvrII C!CTAGG 0 BamHI G!GATCC 2 837 1148 BanI G!GYRCC 5 236 430 923 1512 2609 BanII GRGCY!C 2 996 1127 BbsI !GAAGAC 0 BbsI !GTCTTC 0 BbvI GCAGCNNNNNNNN! 6 54 808 2187 2190 2396 3090 BbvI !NNNNNNNNNNNNGCTGC 9 242 315 441 1254 1579 1660 1678 2097 2699 BclI T!GATCA 3 1092 1131 1385 BfaI C!TAG 7 444 931 987 1195 2263 2516 2851 BglI GCCNNNN!NGGC 3 252 879 2781 BglII A!GATCT 1 906 BpmI !CTGGAG 0 BpmI !CTCCAG 1 2745 Bpu1102I GC!TNAGC 0 BsaA1 YAC!GTR 0 BsaB1 GATNN!NNATC 1 1384 BsaH1 GR!CGYC 3 237 3198 3580 BsaI !NNNNNGAGACC 2 481 2722 BsaI GGTCTCN! 1 1041 BsaJI C!CNNGG 8 355 912 918 919 996 1031 1507 1928 BsgI GTGCAG! 0 BsgI !CTGCAC 1 655 BsiEI CGRY!CG 8 280 688 977 1144 1684 2108 3031 3180 BsiWI C!GTACG 0 BslI CCNNNNN!NNGG 9 54 613 918 1032 1616 1790 1808 1974 2253 BsmAI !GTCTC 3 663 1035 3492 BsmAI !GAGAC 3 51 486 2727 BsmFI !NNNNNGTCCC 1 599 BsmFI GGGACN! 0 BsmI GAATGCN! 1 1204 BsmI G!CATTC 1 1297 Bsp1286I GDGCH!C 8 182 435 577 996 1127 2086 3247 3332 BspDI AT!CGAT 0 BspEI T!CCGGA 0 BspHI T!CATGA 3 2488 3496 3601 BspMI ACCTGCNNNN! 1 1404 BspMI !NNNNNNNNGCAGGT 0 BsrBI CCG!CTC 2 979 1460 BsrBI GAG!CGG 2 1701 3502 BsrDI !CATTGC 1 2896 BsrDI GCAATGNN! 1 2722 BsrFI R!CCGGY 4 683 687 1053 2741 BsrGI T!GTACA 0 BsrI ACTGGN! 4 874 2176 2189 3137 BsrI C!CAGT 10 366 392 398 936 1568 2301 2707 2825 2868 3307 BssHII G!CGCGC 0 Bst1107I GTA!TAC 0 BstBI TT!CGAA 1 1003 BstEII G!GTNACC 0 BstNI CC!WGG 5 356 1508 1796 1917 1930 BstPI G!GTNACC 0 BstUI CG!CG 14 4 6 109 637 738 998 1049 1615 1617 1815 2396 2726 3219 3551 BstXI CCANNNNN!NTGG 2 943 969 BstYI R!GATCY 9 837 906 1148 2409 2420 2506 2518 3286 3303 Bsu36I CC!TNAGG 0 ClaI AT!CGAT 0 DdeI C!TNAG 9 172 818 1114 1121 2043 2452 2618 3158 3584 DpnI GA!TC 22 279 839 844 908 1094 1133 1143 1150 1387 2336 2411 2422 2430 2508 2520 2625 2966 2984 3030 3288 3305 3341 DpnII !GATC 22 277 837 842 906 1092 1131 1141 1148 1385 2334 2409 2420 2428 2506 2518 2623 2964 2982 3028 3286 3303 3339 DraI TTT!AAA 5 1083 1344 2527 2546 3238 DraIII CACNNN!GTG 0 DrdI GACNNNN!NNGTC 2 98 1876 DsaI C!CRYGG 2 912 996 EaeI Y!GGCCR 7 389 685 770 871 974 1607 3049 EagI C!GGCCG 2 685 974 Eam1105I GACNNN!NNGTC 1 2661 EarI !CTCTTC 3 290 1645 3449 EarI !GAAGAG 0 Eco47III AGC!GCT 0 Eco57I !CTGAAG 1 2294 Eco57I !CTTCAG 1 3342 EcoNI CCTNN!NNNAGG 0 EcoO109I RG!GNCCY 2 992 3637 EcoRI G!AATTC 1 947 EcoRII !CCWGG 5 354 1506 1794 1915 1928 EcoRV GAT!ATC 1 959 Esp3I !NNNNNGAGACG 1 46 Esp3I CGTCTCN! 1 669 Fnu4HI GC!NGC 25 43 152 256 329 455 736 797 974 977 1268 1593 1674 1692 1695 1813 1968 2111 2176 2179 2385 2713 3052 3079 3174 3403 FokI GGATGNNNNNNNNN! 3 91 335 437 FokI !NNNNNNNNNNNNNCATCC 4 579 2627 2808 3095 FspI TGC!GCA 3 259 561 2883 HaeII RGCGC!Y 3 240 1646 2016 HaeIII GG!CC 22 289 391 461 503 687 772 873 882 892 917 976 994 1119 1609 1783 1794 1812 2246 2704 2784 3051 3638 HgaI GACGCNNNNN! 2 1879 2457 HgaI !NNNNNNNNNNGCGTC 2 98 3187 HgiAI GWGCW!C 5 182 1127 2086 3247 3332 HhaI GCG!C 18 6 109 239 260 562 1552 1617 1645 1678 1948 2015 2115 2289 2398 2791 2884 3221 3553 HincII GTY!RAC 2 1283 1393 HindIII A!AGCTT 2 650 1409 HinfI G!ANTC 9 708 811 983 1041 1603 1668 1743 2139 2656 HpaI GTT!AAC 1 1283 HpaII C!CGG 17 49 83 500 684 688 919 1054 1486 1975 2122 2148 2338 2742 2776 2843 2953 3195 HphI GGTGANNNNNNNN! 5 25 34 717 3147 3388 HphI !NNNNNNNTCACC 5 1056 1062 2504 2731 3353 KasI G!GCGCC 1 236 KpnI GGTAC!C 1 927 MaeII A!CGT 7 375 411 662 2471 2887 3260 3580 MaeIII !GTNAC 13 57 348 368 439 1253 2124 2187 2303 2586 2917 2975 3128 3316 MboI !GATC 22 277 837 842 906 1092 1131 1141 1148 1385 2334 2409 2420 2428 2506 2518 2623 2964 2982 3028 3286 3303 3339 MboII GAAGANNNNNNNN! 1 2430 MboII !NNNNNNNTCTTC 7 284 624 1639 2501 3256 3334 3443 MluI A!CGCGT 1 1047 MnlI !NNNNNNNGAGG 11 695 982 1138 1318 1327 1616 1875 2199 2599 2680 3627 MnlI CCTCNNNNNNN! 13 40 300 481 903 1036 1041 1109 1148 1368 1667 1950 2828 3034 MscI TGG!CCA 1 772 MseI T!TAA 17 143 340 408 1082 1282 1343 1539 1598 2474 2526 2531 2545 2598 2833 2872 3237 3609 MspI C!CGG 17 49 83 500 684 688 919 1054 1486 1975 2122 2148 2338 2742 2776 2843 2953 3195 MunI C!AATTG 1 1292 NaeI GCC!GGC 2 685 689 NarI GG!CGCC 1 237 NciI CC!SGG 7 49 84 919 920 2148 2844 3195 NcoI C!CATGG 1 912 NdeI CA!TATG 2 185 533 NgoMI G!CCGGC 2 683 687 NheI G!CTAGC 0 NlaIII CATG! 15 42 566 573 916 1160 1407 1426 1772 2492 2983 2993 3071 3107 3500 3605 NlaIV GGN!NCC 17 238 432 478 504 682 839 925 994 1150 1514 1800 1839 2611 2705 2746 2957 3547 NotI GC!GGCCGC 1 974 NruI TCG!CGA 0 NsiI ATGCA!T 1 864 Nsp(7524)I RCATG!Y 4 42 566 1407 1772 NspBII CMG!CKG 8 115 309 998 1090 1592 2110 2355 3296 PacI TTAAT!TAA 0 PflMI CCANNNN!NTGG 0 PleI GAGTCNNNN! 2 991 2147 PleI !NNNNNGACTC 2 1662 2650 PmeI GTTT!AAAC 1 1083 PmlI CAC!GTG 0 Psp1406I AA!CGTT 3 411 2887 3260 PstI CTGCA!G 3 459 956 1401 PvuI CGAT!CG 3 280 1144 3031 PvuII CAG!CTG 2 309 1592 RsaI GT!AC 5 170 541 925 1052 3141 RsrII CG!GWCCG 0 SacI GAGCT!C 1 1127 SacII CCGC!GG 1 999 SalI G!TCGAC 1 1391 SapI !NNNNGAAGAGC 0 SapI GCTCTTCN! 1 1652 Sau3AI !GATC 22 277 837 842 906 1092 1131 1141 1148 1385 2334 2409 2420 2428 2506 2518 2623 2964 2982 3028 3286 3303 3339 Sau96I G!GNCC 10 287 502 916 992 993 2703 2782 2799 3021 3637 ScaI AGT!ACT 1 3141 ScrFI CC!NGG 12 49 84 356 919 920 1508 1796 1917 1930 2148 2844 3195 SfaNI GCATCNNNNN! 8 163 239 601 774 789 1865 2917 3357 SfaNI !NNNNNNNNNGATGC 4 69 199 1226 3108 SfcI C!TYRAG 5 980 1874 2136 2413 3281 SfiI GGCCNNNN!NGGCC 1 879 SfrI !GCCCGGGC 0 SgrAI CR!CCGGYG 0 SmaI CCC!GGG 1 920 SnaBI TAC!GTA 0 SpeI A!CTAGT 1 930 SphI GCATG!C 2 566 1407 SrfI GCCC!GGGC 0 Sse8387I CCTGCA!GG 1 1401 SspI AAT!ATT 1 3465 StuI AGG!CCT 1 1119 StyI C!CWWGG 1 912 SwaI ATTT!AAAT 0 TaqI T!CGA 11 672 711 981 1003 1039 1101 1140 1144 1392 1868 3312 TfiI G!AWTC 5 708 811 1041 1603 1743 Tth111I GACN!NNGTC 0 XbaI T!CTAGA 1 986 XcmI !CCANNNNNNNNNTGG 0 XhoI C!TCGAG 1 980 XmaI C!CCGGG 1 918 XmaIII C!GGCCG 2 685 974 XmnI GAANN!NNTTC 2 628 3260