pMT/V5-His C Sequence Range: 1 to 3534 Enzyme #Cuts Positions AatII 1 3470 AccI 1 1279 Acc16I 3 258 560 2770 Acc65I 1 849 Acc113I 1 3028 AccB1I 5 235 429 849 1399 2496 AccB2I 3 239 1533 1903 AccBSI 4 905 1347 1588 3389 AclNI 1 856 AclWI 10 844 1030 2216 2302 2304 2400 2401 2864 3181 3185 AcsI 3 673 873 1115 AcyI 3 236 3085 3467 AfaI 5 169 540 851 970 3028 AflIII 3 659 965 1655 AgeI 1 971 AhdI 1 2548 AlwI 10 844 1030 2216 2302 2304 2400 2401 2864 3181 3185 Alw21I 4 181 1973 3134 3219 Alw26I 6 45 480 668 959 2609 3385 Alw44I 3 177 1969 3215 AlwNI 1 2071 Ama87I 1 906 ApaLI 3 177 1969 3215 ApoI 3 673 873 1115 AseI 4 405 1426 1485 2720 AsnI 4 405 1426 1485 2720 Asp700I 3 406 627 3147 Asp718I 1 849 AspEI 1 2548 AspHI 4 181 1973 3134 3219 AspS9I 7 286 501 2590 2669 2686 2908 3524 AvaI 1 906 AvaII 2 2686 2908 AviII 3 258 560 2770 BalI 1 771 BanI 5 235 429 849 1399 2496 BbeI 1 239 BbiII 3 236 3085 3467 BbuI 2 565 1294 Bbv12I 4 181 1973 3134 3219 BclI 2 1010 1272 BcnI 5 48 83 2035 2731 3082 BcoI 1 906 BfaI 7 443 840 857 1082 2150 2403 2738 BfmI 6 454 878 1284 1920 2111 2789 BglI 2 251 2668 Bme18I 2 2686 2908 BmyI 6 181 434 576 1973 3134 3219 BpmI 1 2618 Bpu14I 1 921 BsaI 3 480 959 2609 BsaBI 2 841 1271 BsaHI 3 236 3085 3467 BsaJI 4 354 949 1394 1815 BsaMI 2 1091 1184 BsaOI 7 279 687 903 1571 1995 2918 3067 BsaWI 4 971 1861 2008 2839 Bsc4I 9 52 611 922 949 1502 1676 1694 1860 2139 Bse8I 2 841 1271 Bse118I 4 682 686 971 2628 BseDI 4 354 949 1394 1815 BseRI 1 938 BsgI 1 640 Bsh1285I 7 279 687 903 1571 1995 2918 3067 Bsh1365I 2 841 1271 BshNI 5 235 429 849 1399 2496 BsiEI 7 279 687 903 1571 1995 2918 3067 BsiHKAI 4 181 1973 3134 3219 BsiYI 9 53 612 923 950 1503 1677 1695 1861 2140 BslI 9 53 612 923 950 1503 1677 1695 1861 2140 BsmI 2 1091 1184 BsmBI 2 45 668 BsmFI 1 589 BsoBI 1 906 Bsp119I 1 921 Bsp143II 3 239 1533 1903 Bsp1286I 6 181 434 576 1973 3134 3219 BspCI 2 279 2918 BspHI 3 2375 3383 3488 BspLU11I 1 1655 BspMI 1 1291 BsrBI 4 905 1347 1588 3389 BsrBRI 2 841 1271 BsrDI 2 2609 2783 BsrFI 4 682 686 971 2628 BssAI 4 682 686 971 2628 BssKI 10 46 81 353 1393 1681 1802 1815 2033 2729 3080 BssSI 3 1828 3212 3519 BstBI 1 921 Bst2BI 3 1828 3212 3519 BstDEI 8 171 817 1032 1930 2339 2505 3045 3471 BstEII 1 911 BstF5I 7 83 327 429 591 2527 2708 2995 BstH2I 3 239 1533 1903 BstMCI 7 279 687 903 1571 1995 2918 3067 BstNI 5 355 1395 1683 1804 1817 BstOI 5 355 1395 1683 1804 1817 BstPI 1 911 BstSFI 6 454 878 1284 1920 2111 2789 Bst2UI 5 355 1395 1683 1804 1817 BstXI 2 869 895 BstX2I 8 836 1035 2296 2307 2393 2405 3173 3190 BstYI 8 836 1035 2296 2307 2393 2405 3173 3190 BstZI 2 684 900 CbiI 1 921 CciNI 1 900 CfrI 6 388 684 769 900 1494 2936 Cfr10I 4 682 686 971 2628 Cfr13I 7 286 501 2590 2669 2686 2908 3524 Csp6I 5 168 539 850 969 3027 Csp45I 1 921 DdeI 8 171 817 1032 1930 2339 2505 3045 3471 DraI 5 1001 1231 2414 2433 3125 DraII 1 3524 DrdI 2 97 1763 EaeI 6 388 684 769 900 1494 2936 EagI 2 684 900 Eam1104I 3 296 1539 3343 Eam1105I 1 2548 EarI 3 296 1539 3343 EclHKI 1 2548 EclXI 2 684 900 Eco31I 3 480 959 2609 Eco32I 1 885 Eco47I 2 2686 2908 Eco52I 2 684 900 Eco57I 2 2203 3215 Eco64I 5 235 429 849 1399 2496 Eco88I 1 906 Eco91I 1 911 Eco255I 1 3028 EcoO65I 1 911 EcoO109I 1 3524 EcoRI 1 873 EcoRII 5 353 1393 1681 1802 1815 EcoRV 1 885 EheI 1 237 Esp3I 2 45 668 FauI 7 107 117 277 629 1013 1455 1497 FauNDI 2 184 532 FbaI 2 1010 1272 FokI 7 90 334 436 578 2514 2695 2982 FspI 3 258 560 2770 GsuI 1 2618 HaeII 3 239 1533 1903 HgaI 4 97 1766 2344 3074 HgiEI 2 2686 2908 Hin1I 3 236 3085 3467 HincII 2 1170 1280 HindII 2 1170 1280 HindIII 2 649 1296 HinfI 8 707 810 959 1490 1555 1630 2026 2543 HpaI 1 1170 Hsp92I 3 236 3085 3467 KasI 1 235 KpnI 1 853 Ksp22I 2 1010 1272 Ksp632I 3 296 1539 3343 LspI 1 921 MaeI 7 443 840 857 1082 2150 2403 2738 MaeII 6 374 661 2358 2774 3147 3467 MamI 2 841 1271 MboII 8 283 623 1526 2317 2388 3143 3221 3330 MfeI 1 1179 MflI 8 836 1035 2296 2307 2393 2405 3173 3190 MluI 1 965 MluNI 1 771 MroNI 2 682 686 MscI 1 771 MslI 5 531 818 2800 2959 3318 Msp17I 3 236 3085 3467 MspA1I 7 114 308 1008 1479 1997 2242 3183 MspR9I 10 48 83 355 1395 1683 1804 1817 2035 2731 3082 MunI 1 1179 MvaI 5 355 1395 1683 1804 1817 Mva1269I 2 1091 1184 NaeI 2 684 688 NarI 1 236 NciI 5 48 83 2035 2731 3082 NdeI 2 184 532 NgoAIV 2 682 686 NgoMI 2 682 686 NotI 1 900 NspI 4 41 565 1294 1659 NspV 1 921 NspBII 7 114 308 1008 1479 1997 2242 3183 PaeI 2 565 1294 PaeR7I 1 906 PinAI 1 971 PleI 3 1549 2034 2537 Ple19I 2 279 2918 PmeI 1 1001 PshBI 4 405 1426 1485 2720 Psp1406I 2 2774 3147 PspEI 1 911 PstI 3 458 882 1288 PvuI 2 279 2918 PvuII 2 308 1479 RcaI 3 2375 3383 3488 RsaI 5 169 540 851 970 3028 SalI 1 1278 SapI 1 1539 Sau96I 7 286 501 2590 2669 2686 2908 3524 SbfI 1 1288 ScaI 1 3028 ScrFI 10 48 83 355 1395 1683 1804 1817 2035 2731 3082 SduI 6 181 434 576 1973 3134 3219 SfcI 6 454 878 1284 1920 2111 2789 Sfr274I 1 906 SfuI 1 921 SinI 2 2686 2908 SpeI 1 856 SphI 2 565 1294 Sse8387I 1 1288 SspI 1 3352 TaiI 6 377 664 2361 2777 3150 3470 TaqI 9 671 710 907 921 957 1019 1279 1755 3199 TfiI 5 707 810 959 1490 1630 Tsp45I 6 56 367 438 911 2804 3015 TspRI 10 398 404 825 869 900 1096 2347 2496 2601 2948 TthHB8I 9 671 710 907 921 957 1019 1279 1755 3199 VneI 3 177 1969 3215 VspI 4 405 1426 1485 2720 XbaI 1 839 XhoI 1 906 XhoII 8 836 1035 2296 2307 2393 2405 3173 3190 XmaIII 2 684 900 XmnI 3 406 627 3147 The enzymes listed below do not restrict the vector. AatI AccIII AccB7I AfeI AflII AocI Aor51HI ApaI AscI AspI AtsI AvrII AxyI BamHI BanII BanIII BbrPI BbsI Bbv16II BfrI BglII BlnI BlpI BpiI Bpu1102I BpuAI Bsa29I BsaAI BscI Bse21I BseAI BseCI BsePI BsiMI BsiWI Bsp13I Bsp19I Bsp68I Bsp106I Bsp120I Bsp1407I Bsp1720I BspDI BspEI BspTI BspXI BsrGI BssHII BssT1I Bst98I Bst1107I BstDSI BstSNI Bsu15I Bsu36I CelII Cfr9I Cfr42I ClaI CpoI CspI CvnI DraIII DsaI Ecl136II Eco24I Eco47III Eco72I Eco81I Eco105I Eco130I Eco147I EcoICRI EcoNI EcoT14I EcoT22I EcoT38I ErhI Esp1396I FriOI FseI Kpn2I KspI Mph1103I MroI MspCI NcoI NheI NruI NsiI PacI PauI Pfl23II PflMI PmaCI Pme55I PmlI Ppu10I PpuMI PshAI Psp5II PspAI PspALI Psp124BI PspLI PspOMI PstNHI RsrII SacI SacII SexAI SfiI Sfr303I SgfI SgrAI SmaI SmiI SnaBI SplI SrfI SseBI SspBI SstI SstII StuI StyI SunI SwaI Tth111I Van91I Vha464I XcmI XmaI XmaCI Zsp2I