Restriction Search (pUB6/V5-His/lacZ 24-0779) Sequence Range: 1 to 8510 Indication Mode: Actual Cutting Site Enzyme #Cuts Positions AatI 2 672 5497 AatII 3 862 1912 8509 AccI 3 4053 6314 6321 Acc16I 2 1431 7809 Acc65I 2 1235 1469 Acc113I 1 8067 AccB7I 2 2534 2900 AccBSI 10 242 464 1894 3792 3956 4353 4827 6386 6627 8428 AclNI 2 1177 1253 AcsI 6 1195 1270 1785 4293 5154 6233 AfeI 2 73 3124 AflII 1 1054 AflIII 8 2591 3371 3796 4048 4413 5557 6053 6694 AgeI 1 4419 AhdI 1 7587 Alw21I 8 820 1245 3229 3638 3753 7012 8173 8258 Alw26I 7 483 1916 2856 4407 5646 7648 8424 Alw44I 5 816 3634 3749 7008 8254 AlwNI 4 2999 3424 4175 7110 Ama87I 6 576 1089 2627 4070 4354 5519 AocI 1 1513 Aor51HI 2 73 3124 ApaLI 5 816 3634 3749 7008 8254 ApoI 6 1195 1270 1785 4293 5154 6233 AseI 5 5168 5569 6465 6524 7759 AsnI 5 5168 5569 6465 6524 7759 Asp700I 2 5169 8186 Asp718I 2 1235 1469 AspEI 1 7587 AspHI 8 820 1245 3229 3638 3753 7012 8173 8258 AvaI 6 576 1089 2627 4070 4354 5519 AvaII 7 513 730 987 2830 5786 7725 7947 AviII 2 1431 7809 AvrII 2 954 5498 AxyI 1 1513 BalI 1 5630 BamHI 1 1247 BanII 6 1245 3229 4278 4689 4725 5891 BanIII 1 2113 BbeI 3 41 54 985 BbrPI 2 5558 6056 BbsI 5 598 2806 4058 4660 5712 BbuI 3 4671 5246 5318 Bbv12I 8 820 1245 3229 3638 3753 7012 8173 8258 Bbv16II 5 598 2806 4058 4660 5712 BclI 3 2635 4458 5549 BcoI 6 576 1089 2627 4070 4354 5519 BfmI 9 503 1275 1677 4160 4748 5710 6959 7150 7828 BfrI 1 1054 BglI 3 1441 3562 7707 BglII 1 12 BlnI 2 954 5498 BlpI 2 4302 6022 Bme18I 7 513 730 987 2830 5786 7725 7947 BpiI 5 598 2806 4058 4660 5712 BpmI 8 778 1518 2016 2878 4292 6126 6183 7657 Bpu14I 1 4369 Bpu1102I 2 4302 6022 BpuAI 5 598 2806 4058 4660 5712 BsaI 3 483 4407 7648 Bsa29I 1 2113 BsaAI 3 4971 5558 6056 BsaBI 2 2620 5925 BsaMI 1 6263 BsaWI 7 1070 1466 3844 4419 6900 7047 7878 BscI 1 2113 Bse8I 2 2620 5925 Bse21I 1 1513 Bse118I 7 903 2576 4308 4419 4866 6126 7667 BseCI 1 2113 BsePI 3 836 838 2787 BseRI 4 50 4386 5494 6054 BsgI 7 2205 2415 2628 2748 3216 4188 5814 Bsh1365I 2 2620 5925 BsiHKAI 8 820 1245 3229 3638 3753 7012 8173 8258 BsiWI 1 4060 BsmI 1 6263 BsmBI 2 1916 2856 BsoBI 6 576 1089 2627 4070 4354 5519 Bsp19I 2 5405 5625 Bsp68I 1 5858 Bsp106I 1 2113 Bsp119I 1 4369 Bsp1720I 2 4302 6022 BspCI 6 1412 2138 2618 3071 5862 7957 BspDI 1 2113 BspHI 2 7414 8422 BspLU11I 2 4048 6694 BspMI 3 908 2063 3935 BspTI 1 1054 BspXI 1 2113 BsrBI 10 242 464 1894 3792 3956 4353 4827 6386 6627 8428 BsrBRI 2 2620 5925 BsrDI 2 7648 7822 BsrFI 7 903 2576 4308 4419 4866 6126 7667 BssAI 7 903 2576 4308 4419 4866 6126 7667 BssHII 3 836 838 2787 BssSI 4 2346 5801 6867 8251 BssT1I 6 496 659 954 5405 5498 5625 Bst98I 1 1054 Bst1107I 2 4054 6315 BstBI 1 4369 Bst2BI 4 2346 5801 6867 8251 BstDSI 9 45 295 1621 2528 2666 2755 3027 5405 5625 BstEII 1 4359 BstPI 1 4359 BstSFI 9 503 1275 1677 4160 4748 5710 6959 7150 7828 BstXI 3 1266 3509 4126 BstZI 2 4348 5743 Bsu15I 1 2113 Bsu36I 1 1513 CbiI 1 4369 CciNI 1 4348 CelII 2 4302 6022 Cfr9I 1 5519 Cfr10I 7 903 2576 4308 4419 4866 6126 7667 Cfr42I 1 48 ClaI 1 2113 Csp45I 1 4369 CvnI 1 1513 DraI 4 4449 7453 7472 8164 DraII 2 730 5786 DraIII 4 414 2479 4974 6032 DrdI 4 3398 5018 5714 6802 DsaI 9 45 295 1621 2528 2666 2755 3027 5405 5625 EagI 2 4348 5743 Eam1104I 3 1390 6578 8382 Eam1105I 1 7587 EarI 3 1390 6578 8382 Ecl136II 2 1243 3227 EclHKI 1 7587 EclXI 2 4348 5743 Eco24I 6 1245 3229 4278 4689 4725 5891 Eco31I 3 483 4407 7648 Eco32I 1 2402 Eco47I 7 513 730 987 2830 5786 7725 7947 Eco47III 2 73 3124 Eco52I 2 4348 5743 Eco57I 5 1084 2023 5724 7242 8254 Eco72I 2 5558 6056 Eco81I 1 1513 Eco88I 6 576 1089 2627 4070 4354 5519 Eco91I 1 4359 Eco130I 6 496 659 954 5405 5498 5625 Eco147I 2 672 5497 Eco255I 1 8067 EcoICRI 2 1243 3227 EcoNI 1 958 EcoO65I 1 4359 EcoO109I 2 730 5786 EcoRI 2 1270 4293 EcoRV 1 2402 EcoT14I 6 496 659 954 5405 5498 5625 EcoT22I 2 5248 5320 EcoT38I 6 1245 3229 4278 4689 4725 5891 EheI 3 39 52 983 ErhI 6 496 659 954 5405 5498 5625 Esp3I 2 1916 2856 Esp1396I 2 2534 2900 FauNDI 1 4246 FbaI 3 2635 4458 5549 FriOI 6 1245 3229 4278 4689 4725 5891 FspI 2 1431 7809 GsuI 8 778 1518 2016 2878 4292 6126 6183 7657 HgiEI 7 513 730 987 2830 5786 7725 7947 HincII 5 1715 2339 4167 5563 6322 HindII 5 1715 2339 4167 5563 6322 HindIII 1 1229 HpaI 2 1715 2339 KasI 3 37 50 981 KpnI 2 1239 1473 KspI 1 48 Ksp22I 3 2635 4458 5549 Ksp632I 3 1390 6578 8382 LspI 1 4369 MamI 2 2620 5925 MluI 4 2591 3371 3796 4413 MluNI 1 5630 Mph1103I 2 5248 5320 MroNI 2 4866 6126 MscI 1 5630 MslI 7 2629 3186 3578 4124 7839 7998 8357 MspCI 1 1054 Mva1269I 1 6263 NaeI 2 4868 6128 NarI 3 38 51 982 NcoI 2 5405 5625 NdeI 1 4246 NgoAIV 2 4866 6126 NgoMI 2 4866 6126 NotI 1 4348 NruI 1 5858 NsiI 2 5248 5320 NspI 5 4052 4671 5246 5318 6698 NspV 1 4369 PaeI 3 4671 5246 5318 PaeR7I 1 4354 PauI 3 836 838 2787 Pfl23II 1 4060 PflMI 2 2534 2900 PinAI 1 4419 PleI 9 148 205 587 4260 5027 5035 6588 7073 7576 Ple19I 6 1412 2138 2618 3071 5862 7957 PmaCI 2 5558 6056 PmeI 1 4449 Pme55I 2 672 5497 PmlI 2 5558 6056 Ppu10I 2 5244 5316 PpuMI 2 730 5786 PshBI 5 5168 5569 6465 6524 7759 Psp5II 2 730 5786 Psp1406I 2 7813 8186 PspAI 1 5519 PspALI 1 5521 Psp124BI 2 1245 3229 PspEI 1 4359 PspLI 1 4060 PstI 1 1279 PvuI 6 1412 2138 2618 3071 5862 7957 PvuII 6 1381 3938 4301 4717 5834 6518 RcaI 2 7414 8422 SacI 2 1245 3229 SacII 1 48 SalI 1 6320 SapI 1 6578 ScaI 1 8067 SexAI 1 5265 SfcI 9 503 1275 1677 4160 4748 5710 6959 7150 7828 Sfr274I 1 4354 Sfr303I 1 48 SfuI 1 4369 SinI 7 513 730 987 2830 5786 7725 7947 SmaI 1 5521 SpeI 2 1177 1253 SphI 3 4671 5246 5318 SplI 1 4060 SseBI 2 672 5497 SspI 2 2519 8391 SstI 2 1245 3229 SstII 1 48 StuI 2 672 5497 StyI 6 496 659 954 5405 5498 5625 SunI 1 4060 Van91I 2 2534 2900 Vha464I 1 1054 VneI 5 816 3634 3749 7008 8254 VspI 5 5168 5569 6465 6524 7759 XcmI 2 3506 4251 XhoI 1 4354 XmaI 1 5519 XmaIII 2 4348 5743 XmaCI 1 5519 XmnI 2 5169 8186 Zsp2I 2 5248 5320 There are no restriction sites for the following enzymes: AccIII ApaI AscI AspI AtsI BseAI BsiMI Bsp13I Bsp120I Bsp1407I BspEI BsrGI BstSNI CpoI CspI Eco105I FseI Kpn2I MfeI MroI MunI NheI PacI PshAI PspOMI PstNHI RsrII SbfI SfiI SgfI SgrAI SmiI SnaBI SrfI Sse8387I SspBI SwaI Tth111I XbaI